(no subject)

[enismirdal]

I hate microarrays. Mainly because I never really understood what they were, or how they worked, and because they're so new and cutting edge that no textbook seems to have anything on them, and the websites I've looked at never seem to go into enough detail to be helpful.

I've worked now. I'm stopping for today.

Comments

[cobalt-skye.livejournal.com]

microarrays:
you get your little chip, onto which you put dots of DNA or RNA. the point is to test whether certain genes are being expressed in certain body tissues, so each dot is from a different part of the body. then you have a probe which hybridises to the protein product of the gene. probe the samples- the probes have flourescent tags. thus you end up with little spots of flourescence where the gene is used in the sample.

and if i could find my actual notes on this it would make life soooo much easier! you may actually not have DNA/RNA samples, rather cytoplasmic samples to test for the proteins. yes, actually that prob makes more sense...

[emperor]

You could try asking sphyg, she knows a lot about them.

[talullahred.livejournal.com]

Umm, that was pretty clear to me.
Thanks I was needing some refreshing myself.

[enismirdal.livejournal.com]

Sweet :D

That's brilliant, thanks. *big happy grin*

Oh, and what exactly do they mean by hybridisation? And is the in situ hybridisation they do with microsatellites the same, or different, do you know?

[cobalt-skye.livejournal.com]

hybridisation is always the same as far as i know- in situ means it just occurs in the place you're experimenting in. hybridisation is basically when one molecule binds to another because they are complementary or have the correct binding sites. summat like that

no probs!

[cobalt-skye.livejournal.com]

no problems!
:-)

[cobalt-skye.livejournal.com]

in situ hybridisation uses diff fluorescent tags for diff porbes and its not arrnged neatly in an array. thats about all the diff i think-found it in cells rev notes last night and now lost it again...

[enismirdal.livejournal.com]

Wow, that's wonderful. Thanks, luv! :D :D